Analysis and Comparison of Serum Proteomics of Senescence Accelerated Mice

Chem. J. Chinese Universities

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Analysis and Comparison of Serum Proteomics of Senescence Accelerated Mice

JIANG Ning¹, ZHOU Wen-Xia¹, ZHANG Yong-Xiang¹, ZHANG Xue-Min², WANG Jie², LIU Bing-Yu²

1. Institute of Pharmacology and Toxicology, Chinese Academy of Military Medicine;
2. National Center of Biomedical Analysis, Beijing 100850, China

Received:2005-09-06 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
Contact: ZHOU Wen-Xia

Abstract

Abstract: To analyze and compare differential serum proteins of senescence accelerated mice: SAM-prone/8(SAMP 8) and SAM-resistance/1(SAMR 1), the proteins of sera from 6 and 12 month-old SAMP 8 and age-matched SAMR 1 were separated, detected and analyzed. Compared with the same age SAMR 1, 15 proteins expressions in sera of six-month-old SAMP 8 were up-regulated, 3 proteins expressions down-regulated significantly and 7 proteins only expressed in SAMP 8 sera; 9 proteins expressions in sera of twelve-month-old SAMP 8 were up-regulated, 7 proteins expressions down-regulated significantly and 12 proteins only expressed in SAMP 8 sera. Using MALDI-TOF-MS, 19 proteins with significant changes were identified by peptide fingerprinting-map and the results searched in MASCOT database. The results showed that 6 proteins were only expressed in sera of six-month-old SAMP 8, 4 proteins only expressed in sera of twelve-month-old SAMP 8. In addition, there were 9 common proteins presenting both in sera of six and twelve-month-old SAMP 8. Our study showed that there were significant differences in serum protein expressions between SAMP 8 and SAMR 1, and some differential proteins may result in pathological changes during the course of aging.

Key words: Accelerated senescence mouse, Serum proteomics, Two dimensional gel electrophoresis

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JIANG Ning¹, ZHOU Wen-Xia¹, ZHANG Yong-Xiang¹, ZHANG Xue-Min², WANG Jie², LIU Bing-Yu². Analysis and Comparison of Serum Proteomics of Senescence Accelerated Mice[J]. Chem. J. Chinese Universities, doi: .

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Analysis and Comparison of Serum Proteomics of Senescence Accelerated Mice

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