Chem. J. Chinese Universities

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Studies on Determination of Percentage Bound of Eight Antibiotics by Using a New Home-made Surface Plasomon Resonace Sensor

ZOU Xiang-Yu, ZHOU Jian-Guang, XIE Ping, ZHANG Lei, WANG Shu-Hua,
FENG Guo-Dong, JIN Qin-Han*   

  1. Institute for Miniature Instrumentation, College of Chemistry, Jilin Province
    Research Center for Enginerring and Technology of Spectral Instruments, Jilin University, Changchun 130021, China
  • Received:2005-10-08 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: JIN Qing-Han

Abstract: Based on surface plasmon resonance (SPR), a novel optical chemical sensor was developed. Traditionally, wavelength modulation SPR sensing method adapted tungsten lamp as the light source with an expensive and complicated apparatus. The device presented here is a relatively simple and inexpensive one. It was designed using a white LED with a 5 V battery as the light source. The percentage bound of 8 antibiotics with BSA was studied by this setup. The results were compared with the reliable percentage bound data obtained by standard equilibrium dialysis and a relative good agreement was achieved.

Key words: Surface plasomon resonance sensor, Antibiotic, Protein, Percentage bound

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