CJCU

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (1): 79.

• Contents • Previous Articles     Next Articles

Proteomic Analysis of Proteins with Altered Expression Induced
by Phenylephrine in Neonatal Rat Cardiomyocyte

LIU Ning1,  LI Zi-Jian 2,  LIU Zhi-Qiang1,  LIU Shu-Ying2,  HAN Qi-De1,  ZHANG You-Yi2   

  1. 1.  Laboratory of New Drug Research and Mass Spectrometry,  Changchun Institute of Applied Chemistry, 
    Chinese Academy of Sciences,  Changchun Center of Mass Spectrometry,  Changchun 130022,    China;
    2.  Institute of Vascular Medicine,  Third Hospital, Key Laboratory of
    Molecular Cardiovascular Sciences of  Ministry of Education,  Peking University, Beijing 100083,   China
  • Received:2005-10-08 Online:2006-01-10 Published:2006-01-10
  • Contact: LIU Zhi-Qiang,E-mail:liuzq@ciac.jl.cn ZHANG You-Yi,E-mail:zhangyy@bjmu.edu.cn

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Abstract:

AbstractIn order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE),   the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by  using a series of methods,  including two dimensional polyacrylamide gel electrophoresis,  PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.  The good two-dimensional  pattern with a high resolution and reproducibility was obtained.  Totally 14 differential protein spots were found.  Out of these,  11 proteins were preliminarily identified. Desmin, Txndc5, etc. were found to be up-regulated in PE-treated cardiomyocytes, while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase, etc. were found to be down-regulated. It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.

Key words: Phenylephrine; Cardiomyocyte; Proteome

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