Analyses of Seven Kinds of Phenolic Acids in Wine by HPLC with Electrochemical Detection

Abstract

Abstract: A rapid, sensitive and accurate reverse phase HPLC-ECD method for simultaneous determination of seven kinds of bioactive phenolic acids(gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid) in five different types of wine was developed. The separation was performed on Hypersil ODS column(250 mm×4.0 mm, 5.0 μm) by gradient elution. The mobile phase consisted of CH-3OH-4%HAc. The flow rate was 0.8 mL/min. The working potential was 0.7 V and column temperature was 30 ℃. The experimental results showed that the seven kinds of phenolic acids were separated satisfactorily. The detection limit of phenolic acids with ECD was 4-600 times greater than those obtained with UV detection.

Key words: HPLC, Electrochemical detection, Wine, Phenolic acid

CLC Number:

O657

TrendMD:

CAO Wei, SUO Zhi-Rong, SONG Ji-Rong, ZHENG Jian-Bin . Analyses of Seven Kinds of Phenolic Acids in Wine by HPLC with Electrochemical Detection[J]. Chem. J. Chinese Universities, 2005, 26(8): 1424.

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Analyses of Seven Kinds of Phenolic Acids in Wine by HPLC with Electrochemical Detection

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