Preparation of a Novel DNA Electrochemical Sensor and Its Application to Detection of DNA Oxidative Damage

Abstract

Abstract: A novel DNA electrochemical sensor was prepared by immobilizing dsDNA on the hydroxyapatite(HAp)-polyvinyl alcohol(PVA) coating which was prepared by sol-gel methold on the glass carbon electrode surface.The sensor was used to detect the oxidative damage to dsDNA mediated by Fe²⁺ Fenton reaction.The results clearly indicate that the DNA damage can be accelerated by the presence of excessive AA in this experiment.The lower concentration of EDTA and the relative higer concentration of Fe²⁺ in the Fenton reaction solution can produce more OH·,and then result in the deeper damage of DNA.

Key words: DNA electrochemical sensor, Detection of DNA damage, Hydroxyapatite, Fenton reaction

CLC Number:

O657.1

TrendMD:

WANG Gui-Xiang, PAN Qian-Xiu, WANG Huai-Sheng . Preparation of a Novel DNA Electrochemical Sensor and Its Application to Detection of DNA Oxidative Damage[J]. Chem. J. Chinese Universities, 2005, 26(10): 1812.

References

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