Construction and Performance of Two-dimensional Separation Platform of Coupling Capillary Isoelectric Focusing-zone Electrophoresis for Proteins

Chem. J. Chinese Universities ›› 2004, Vol. 25 ›› Issue (8): 1451.

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Construction and Performance of Two-dimensional Separation Platform of Coupling Capillary Isoelectric Focusing-zone Electrophoresis for Proteins

LIU He-Chun, YANG Chun, ZHANG Wei-Bing, ZHANG Yu-Kui

National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China

Received:2003-05-19 Online:2004-08-24 Published:2004-08-24

Abstract

Abstract: A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.

Key words: Microialysis hollow fiber membrane interface, Two-dimensional separation platform, Capillary isoelectric focusing, Capillary zone electrophoresis

CLC Number:

O658

TrendMD:

LIU He-Chun, YANG Chun, ZHANG Wei-Bing, ZHANG Yu-Kui . Construction and Performance of Two-dimensional Separation Platform of Coupling Capillary Isoelectric Focusing-zone Electrophoresis for Proteins[J]. Chem. J. Chinese Universities, 2004, 25(8): 1451.

[1]	ZHU Gui-Jie, YANG Chun, LIU He-Chun, ZHANG Li-Hua, ZHANG Wei-Bing, ZHANG Yu-Kui. Construction of a New Method for Mobilizing the Zones of CIEF and Its Application [J]. Chem. J. Chinese Universities, 2005, 26(4): 654.
[2]	WANG Zong-Hua, YAN Liu-Shui, LUO Guo-An. Simultaneous Determination of Inorganic Anions and Cations in Cosmetics by Capillary Zone Electrophoresis [J]. Chem. J. Chinese Universities, 2001, 22(S1): 37.
[3]	CAO YuHua, ZHANG Xin, FANG YuZhi, YE JianNong . Determination of Phenolic Compounds in Red Wine by Capillary Zone Electrophoresis with Electrochemical Detection [J]. Chem. J. Chinese Universities, 2001, 22(12): 2011.
[4]	CHEN Gang, DING Xiang-Huan, YE Jian-Nong . Determination of Rutin and L-Ascorbic Acid in Pharmaceutical Preparations and Fruit Juices by Capillary Zone Electrophoresis with Electrochemical Detection [J]. Chem. J. Chinese Universities, 2000, 21(9): 1364.
[5]	ZHANG Hong-Yi, LI Qian-Feng, LIU Hui-Tao, CHEN Xing-Guo-HU Zhi-De, WANG Rui. The Investigation of a New Recording Mode for Capillary Zone Electrophoresis [J]. Chem. J. Chinese Universities, 2000, 21(12): 1832.
[6]	TIAN Xiao-Dan, YANG Peng-Yuan, HE Xing-Cun, WANG Xiao-Ru . Studies on Speciation of Arsenious Compounds by Capillary Zone Electrophoresis [J]. Chem. J. Chinese Universities, 1998, 19(1): 27.
[7]	HE Jin-Lan, LI Hui-Ping, Ll Xiao-Ge. Quantitative Analysis of Prostaglandins in SD Rats by Capillary Zone Electrophoresis [J]. Chem. J. Chinese Universities, 1997, 18(11): 1797.
[8]	ZHAO TaO, LIU Qi-Ping, CHENG Jie-Ke. Capillary Zone Electrophoresis Separation of Nine Purine Bases [J]. Chem. J. Chinese Universities, 1996, 17(6): 883.
[9]	LIN Bing-Cheng, SHI Wei, HU Liu-Jiang, YANG Guo-Liang, HUANG Jian-Fu, ZHANG Nai-Qian. The Effect of the Buffer pH in Capillary Zone Electrophoresis [J]. Chem. J. Chinese Universities, 1993, 14(9): 1229.

Construction and Performance of Two-dimensional Separation Platform of Coupling Capillary Isoelectric Focusing-zone Electrophoresis for Proteins

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