Abstract: IgA nephropathy is a kidney disorder caused by deposits of IgA inside the glomeruli of the kidney. This chronic disease may progress over a period of 10 to 20 years and usually cannot be cured effectively. Immunoadsorbent can remove the excess IgA by hemoperfusion. We prepared the adsorbent by reacting agarose with epoxy chloropropane to introduce the active epoxy group. Then it was reacted with ammonium hydroxide and glutaraldehyde to introduce a spacer and sheep anti-human IgA antibody was linked to the spacer as a ligand. In vitro experiments showed that the adsorbent thus prepared could remove excess IgA in plasma effectively and the adsorption capacity reached 74%.

Key words: IgA-nephropathy, Agarose, Immnoadsorbent