Application of Comprehensive Two-dimensional Gas Chromatography/ Time-of-flight Mass Spectrometry to Analysis of Zedoary Volatile Oil

Abstract

Abstract: After optimizing the chromatographic conditions including column system,temperature program and modulation parameters,an analytical method of zedoary volatile oil was established based on comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry(GC×GC/TOFMS). The analysis of the individual components and groups were achieved. Under the optimized conditions the components of zedoary volatile oil from Guangxi province,China were identified by GC×GC/TOFMS. Among 249 components with similarities over 800,there are 18 terpenes,34 oxygenated terpenes,35 sesquiterpenes and 37 oxygenated sesquiterpenes. Sixty-nine of these components have the individual volume fractions of over 0.02%.

Key words: Volatile oil, Zedoary, Column system, Comprehensive two-dimensional gas chromatography, Time-of-flight mass spectrometry

CLC Number:

O655

TrendMD:

WU Jian-Fang, LU Xin, TANG Wan-Ying, LIAN Xiao-Hong, KONG Hong-Wei, RUAN Chun-Hai, XU Guo-Wang . Application of Comprehensive Two-dimensional Gas Chromatography/ Time-of-flight Mass Spectrometry to Analysis of Zedoary Volatile Oil[J]. Chem. J. Chinese Universities, 2004, 25(8): 1432.

References

[1] 　R uan C.H.,Xu G.W.,L u X.et al..Ch rom atog rap h ia,2003,57:1—6 [2] 　L iu Z.,Ph illip s J.B..J.Ch rom atog r.Sc.i[J],1991,29:227—231 [3] 　V enkatram an i C.J.,Xu J.,Ph illip s J.B..A nal.Chem.[J],1996,68:1 486—1 492 [4] 　XU Guo-W ang(许国旺),YE Fen(叶芬),KON G Hong-W ei(孔宏伟)et al..Ch rom atog rap hy(色　谱)[J],2001,19(2):132—136 [5] 　M arrio tt P.,Shellie R.,Co rnw ell C..J.Ch rom atog r.A.[J],2001,936:1—22 [6] 　Been s J.,Boelenn s H.,T ijssen R.et al..J.H igh R eso l.Ch rom atog r.[J],1998,21:47—54 [7] 　de Geu s H.,de Boer J.,Ph illip s J.B.et al..J.H igh R eso l.Ch rom atog r.[J],1998,21:411—413 [8] 　D eu rsen M.van,Been s J.,R eijenga J.et al..J.H igh R eso l.Ch rom atog r.[J],2000,23(7 8):507—510 [9] 　D alllüge J.,R ijn M.van,Been s J.et al..J.Ch rom atog r.A.[J],2002,965:207—217 [10] 　Shellie R.,M arrio tt P.,M o rrison P..A nal.Chem.[J],2001,73:1 336—1 344 [11] 　FU N ai-W u(傅乃武).Ch in.M ed.Bu ll.(中药通报)[J],1984,9(2):35—38 [12] 　ZHOU X in(周　欣),L IAN G Guang-Y i(梁光义),SH EN W an-Yan(沈万雁)et al..W est Ch ina J.Pharm.Sin.(华西药学杂志)[J],2002,17(3):201—203 [13] 　L I A i-Q un(李爱群),HU Xue-J un(胡学军),D EN G Yuan-H u i(邓远辉)et al..Ch in.T rad it.H erb D rug(中草药)[J],2001,32(9):782—783 [14] 　TAN G M in-Yan(汤敏燕),SU N L ing-Feng(孙凌峰),W AN G Hong-W u(汪洪武).Chem.Indu s.Fo rest P rod.(林产化学与工业)[J],2000,20(3):65—69 [15] 　SH EN Sh i-J ie(沈世杰),HAN J iu-M an(韩纠缦).Ch in.T rad it.H erb D rug(中草药)[J],1997,28(1):10—13 [16] 　W AN G Xue-Feng(王雪峰),YAO Chuan(姚川),HU Chang(胡昌).J.Ch in.M ed.M ater.(中药材)[J],1991,14(10):35—37 [17] 　Pharm acopoeia of Peop le′s R ep ub lic of Ch ina(中华人民共和国药典)[M],Beijing:Chem ical Indu stry P ress,1995,A pp end ix:62 [18] 　O ng R.,M arrio tt P.,M arrison P..J.Ch rom atog r.A.[J],2002,962:135—152 [19] 　O ng R.C.Y.,M arrio tt P.J..J.Ch rom atog r.Sc.i[J],2002,40:276—291 [20] 　Been s J.,B lom berg J.,Schoenm akers P.J..J.H igh R eso l.Ch rom atog r.[J],2000,23:182—188