Stroma-free Hemoglobin Cross-linked with O-Raffinose and Glutaraldehyde

Abstract

Abstract: Red cell substitutes are group of oxygen carriers designed to temporarily replace transfused blood. In this paper, a kind of red cell substitutes based on polymerized hemoglobin were prepared and in vitro characteristics were studied. Firstly, Stroma-free hemoglobin(SFHb) was purified from outdated human blood. SFHb was intramolecularly crosslinked with O-Raffinose, prepared from the ring opening oxidation of raffinose. The intramolecular crosslinking between amino groups within 2,3-DPG binding pocket contributes to high P₅₀. Then O-Raffinose-Hb was intermolecularly crosslinked with glutaraldehyde under the optimized crosslinking condition. The process of crosslinking was monitored by SDS-PAGE and Sepharose gel chromatography. The percentage of the component of polyhemoglobin with a molecular weight range of 130 000—600 000 was 76.9%.

Key words: Stroma-free hemoglobin, O-Raffinose, Glutaraldehyde, Molecular weight distribution, Polyhemoglobin

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WANG Yan-Feng, PAN Ji-Lun, YU Yao-Ting . Stroma-free Hemoglobin Cross-linked with O-Raffinose and Glutaraldehyde[J]. Chem. J. Chinese Universities, 2004, 25(10): 1845.

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Stroma-free Hemoglobin Cross-linked with O-Raffinose and Glutaraldehyde

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