Abstract: Microfluidic glass chips with integrated ultra-micro electrodes were fabricated. In pattern EC-1, a 13-μm-wide platinum electrode was located photolithographically at the end of separation channel and extended 30 μm into the 6 mm wide detection reservoir. The electrode system and separation voltage were optimized, and neurotransmitters were detected after electrophoretic separation. In pattern EC-2, a 7-μm-wide platinum electrode with a battlement-like membrane structure were integrated in the chips. With post-etching procedure, the membrane width was reduced to 10 μm. With good electric conductivity and heat dissipation capability, the porous membrane successfully shields the high electric field before the ultra-micro electrode, which shows great possibility to apply in the on-chip electrochemical detection.

Key words: Microfluidic chips, Ultra-micro electrode, Electrochemical detection, Porous membrane