Chemical Modification of Abzyme with Deiodinase Activity

Abstract

Abstract: The abzyme with deiodinase activity was modified with diethyl pyrocarbonate(DEPC) and carbodiimide(EDC) respectively. The modified abzymes were dialyzed and their activities were compared with that of the native abzyme. The results indicate that the histidine residue is one of the essential groups in the abzyme, and the modification causes the catalytic activity a loss of 70%. The modification of the residues containing carboxyl group with EDC leads to a loss of 50% activity, indicating that acid amino residues play important roles in the catalytic reaction.

Key words: Essential groups, Chemical modification, Abzyme

CLC Number:

Q553

TrendMD:

SUN Li Wei, LIU Li Yan, ZHAO Da Qing. Chemical Modification of Abzyme with Deiodinase Activity[J]. Chem. J. Chinese Universities, 2003, 24(4): 588.

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Chemical Modification of Abzyme with Deiodinase Activity

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