Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (3): 390.
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ZHANG Li-Ping1,2, YANG Pin1
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Abstract: The action mechanism of Cu 2+ to Slow Vacuolar(SV) channels of Radish(Raphanus satirus L.) was investigated by using whole-vacuolar patch-clamp recording mode. It is shown that SV channel was a cation selective channel and it had voltage- and time-dependence. Cytoplasmic Ca 2+ could activate SV channel and the increase in cytoplasmic Ca 2+ led to enhancement of SV-type currents. The changes of SV current was shown after Cu 2+ with different concentrations was added to the bath. When CuCl2 with different concentrations was added to the bath solution, SV currents have different effects. It was found that CuCl2 with a lower concentration(<0.1 mmol/L) promoted the channel currents, while CuCl2 with a higher concentration(>0.1 mmol/L) inhibited SV currents. When 1 μmol/L CuCl2 was added to pipette solution, SV currents were inhibited remarkably. This result provides an important basis at the channel level for researching further the effects of Cu 2+ on physiological activities of plants and the effects of copper fertilizers on crops.
Key words: Whole-vacuolar recording, SV-type current, Cytoplasmic Ca2+, Cu 2+, Patch-clamp
CLC Number:
O614.33
TrendMD:
ZHANG Li-Ping, YANG Pin . Effects of CuCl2 on Slow Vacuolar Ion Channels in Radish by Patch-clamp[J]. Chem. J. Chinese Universities, 2003, 24(3): 390.
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http://www.cjcu.jlu.edu.cn/EN/Y2003/V24/I3/390