Catalytic Hydrolysis and Modification of Microporous Nylon-6 Membranes and Insite Synthesis of DNA Chip Active Membranes

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (11): 2089.

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Catalytic Hydrolysis and Modification of Microporous Nylon-6 Membranes and Insite Synthesis of DNA Chip Active Membranes

TANG Jian-Xin, LIU Zheng-Chun, LI Song, NIE Li-Bo, XIAO Peng-Feng, HE Nong-Yue

Key Lab for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China

Received:2002-10-10 Online:2003-11-24 Published:2003-11-24

Abstract

Abstract: Microporous polyamide-6 membranes were hydrolyzed in 0.01mol/L NaOH/CH₃OH medium by refluxing for about 36h.By investigating the UV spectra, the hydrolyzing conditions were optimized.ApH value of 12 and reaction time of 36h are the preferred conditions for hydrolysis.The treated membrane can be applied for in site synthesis of oligonucleotide and, for example, the oligonucleotide probes of 5′-AACCACCAAACACAC-3′ were successfully synthesized on the hydrolyzed membrane,which was proved by the high resolution X-ray photoelectron spectroscopy(XPS) of P_2pThe single step coupling efficiency was determined by UVspectra to be in excess of 98%.

Key words: Catalytic hydrolysis, Amino group, Nylon-6 membrane, DNA in site synthesis, Coupling efficiency

CLC Number:

O641

TrendMD:

TANG Jian-Xin, LIU Zheng-Chun, LI Song, NIE Li-Bo, XIAO Peng-Feng, HE Nong-Yue . Catalytic Hydrolysis and Modification of Microporous Nylon-6 Membranes and Insite Synthesis of DNA Chip Active Membranes[J]. Chem. J. Chinese Universities, 2003, 24(11): 2089.

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Catalytic Hydrolysis and Modification of Microporous Nylon-6 Membranes and Insite Synthesis of DNA Chip Active Membranes

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