Isolation and Characterization of Toxin Polypeptides fromSea Anemone Actinia cari

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (4): 605.

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Isolation and Characterization of Toxin Polypeptides fromSea Anemone Actinia cari

ZHUO Ru-Peng¹, FU Hong-Zheng¹, LI Jun¹, ZHANG Li-He¹, ZHONG Chong-Bo², WANG Xiao-Liang², LIN Wen-Han¹

1. National Key Laboratory of Natural and Biomimetic Drugs, Peking Beijing 100083, China;
2. Institute of Materia Chinese Academy of Medicad Sciences, Beijing 100050, China

Received:2000-12-15 Online:2002-04-24 Published:2002-04-24

Abstract

Abstract: Two toxin polypeptides desgnated RSAPⅢ and RSAP Ⅳ were isolated from sea anemone Ac-tinia cari.Toxins isolation was accomplished by freezing-thawing extraction, fractional acetone precipita-tion, cation exchange chromatography on SP Sephadex C-25 and reverse phase HPLC on ODS C₁₈. Theywere found to be homogenous by SDS-PAGE, and their moleculax weight at m/z 5327 and m/z 5310 re-spectively were determined by MALDI-TOF-MS.RSAP Ⅲ possessed a bioactivity on sodinm channel inGuinea pig cardiac cell to inhibit inward Na⁺ currents, which is contrary to that of all sea anemone toxinsisolated so far.

Key words: Actinia cari, Sea anemone toxin, Isolation and purification

CLC Number:

O629.72

TrendMD:

ZHUO Ru-Peng, FU Hong-Zheng, LI Jun, ZHANG Li-He, ZHONG Chong-Bo, WANG Xiao-Liang, LIN Wen-Han. Isolation and Characterization of Toxin Polypeptides fromSea Anemone Actinia cari[J]. Chem. J. Chinese Universities, 2002, 23(4): 605.

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Isolation and Characterization of Toxin Polypeptides fromSea Anemone Actinia cari

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