Layer-by-Layer Assembly of Multilayer Films Composed of Streptavidin and Biotinylated Antibody by Real-time Biomolecular Interaction Analysis

Abstract

Abstract: Layer-by-layer assembly of multilayer films of streptavidin and biotinylated antibody was completed on the streptavidin coated surface. Real-time biomolecular interaction analysis(BIA) based on surface plasmon resonance technique was used to monitor the multilayer assembly in solution continuously. The results indicate that the uniform multilayer film can be fabricated successfully based on the strong interaction between streptavidin and biotin. The mean surface mass concentration of each adsorption layer is 1.32 ng/mm² for biotinylated antibody, 2.93 ng/mm² for streptavidin, according to the correlation of SPRresponse with surface concentration.

Key words: Surface plasmon resonance, Real-time biomolecular interaction analysis(BIA), Layer-by-layer assembly, Streptavidin, Biotinylated antibody

CLC Number:

O657

TrendMD:

PEI Ren-Jun, CUI Xiao-Qiang, YANG Xiu-Rong, WANG Er-Kang . Layer-by-Layer Assembly of Multilayer Films Composed of Streptavidin and Biotinylated Antibody by Real-time Biomolecular Interaction Analysis[J]. Chem. J. Chinese Universities, 2002, 23(2): 195.

References

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