Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (8): 1349.

• Articles • Previous Articles     Next Articles

Studies on the Cleavage of Bovine Serum Albumin by Ser-His

CHEN Jing, WAN Rong, LIU Hai, JIANG Yu-Yang, ZHAO Yu-Fen   

  1. The Key Laboratory of Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China
  • Received:2000-06-08 Online:2001-08-24 Published:2001-08-24

Abstract: It has been found that Ser-His could cleave bovine serum albumin(BSA), with the most suitable pHaround 6.0. The kinds of buffers have different influences on the cleavage activity. The phosphate buffer and BRbuffer are the most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and carboxyl group of Ser-His increase the cleavage activity.

Key words: Seryl histidine dipeptide, Bovine serum albumin(BSA), Protein cleavage

CLC Number: 

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