CJCU

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (6): 922.

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Spectroscopic Characteristics of RTP Palladium-porphyrin Probe Interacting with DNA in Aqueous Solution

YUAN Wen1, JIN Wei-Jun2, DONG Chuan2   

  1. 1. Department of Chemistry, Normal College, Shanxi University, Taiyuan 030012, China;
    2. Department of Chemistry, Shanxi University, Taiyuan 030006, China
  • Received:2000-04-27 Online:2001-06-24 Published:2001-06-24

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Abstract: The characteristics of absorption, fluorescence, room temperature phosphorescence(RTP) spectra of meso tetrakis(4-N-trimethylaminobenzyl)porphin(TAPP) and Palladium porphyrin(Pd-TAPP) probe in the presence and absence of ct-DNAhas been studied. Pd-TAPP shows a very weak RTPemission in aqueous solution and strong RTP emission in the presence of DNA. Maximum RTPwas observed at pH=7, with maximum excitation and emission wavelengths at 414 nm and 684 nm, respectively. RTPdecay follows a first order exponential equation, RTPlifetime (τ) of probe is (0.72±0.03)ms. The detection limit of ct-DNA may reach 8.8×10-7 mol/L. The relative standard deviation(RSD) is less than ±6.6% in the linear range.

Key words: Palladium-porphyrin, Room temperature phosphorescence probe, ct-DNA

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