Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (5): 754.

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Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay

YANG Huang-Hao¹, ZHU Qing-Zhi¹, LI Dong-Hui², DING Ma-Tai¹, XU Jin-Guo¹

Department of Chemistry, the Key Laboratory of Analytical Sciences of MOE, Cancer Rechearch Center, Xiamen University, Xiamen 361005, China

Received:2000-06-09 Online:2001-05-24 Published:2001-05-24

Abstract

Abstract: Atemperature sensitive polymer containing a terminal carboxyl group was prepared by copolymerization of N-isopropylacrylamide with mercaptoacetic acid. This polymer was conjugated to the IgGwith EDCIand used to develop a novel polymer mimetic enzyme competitive immunoassay method for determination of anti-IgGwith iron-tetrasulfonatophthalocyanine as a labelling reagent to catalyze the reaction of p-hydroxyphenyl acetic acid (HPA) and hydrogen peroxide in alkaline medium. The calibration graph for anti-IgGwas linear over the range of 0-1 500 ng/mLwith a detection limit of 2.0 ng/mL.

Key words: Oligo N-isopropylacrylamide, Temperature sensitive polymer, Heat sensitive phase separation, Mimetic enzyme, Fluoroimmunoassay

CLC Number:

O657.3

TrendMD:

YANG Huang-Hao, ZHU Qing-Zhi, LI Dong-Hui, DING Ma-Tai, XU Jin-Guo . Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay[J]. Chem. J. Chinese Universities, 2001, 22(5): 754.

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Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay

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