The Biological Activities of the Small Peptide Immunosuppressant

Abstract

Abstract: According to the previous studies, in which we used a monoclonal antibody 5G1 that is specific for the human IL 2 receptor α chain to screen a random hexa peptide library and obtained the phage clone which has good combinative activities studied by Elisa, the consensus sequence motif C-S-Swas revealed by DNAsequencing, the peptide Ac XCSSXX NH₂ was synthesized, then purified by HPLCand confirmed by MS. To investigate the biological activities of the hexa peptide, we constructed the vitro model of the lymphocyte proliferation experiment(LPE) induced by ConAand IL 2 and the vivo model with liver transplanting of rat. In the LPEinduced by ConA, the addition of hexa peptide(250-3125 μg/mL) had a significant inhibitory effect to LPE( P-001). The minimal inhibitory mass concentration is 3125 μg/mL, with the inhibitory rate of 78.16%. In the LPEinduced by IL 2, we obtained the same result as the LPEinduced by ConA. So we can say that the peptide realizes its immuno suppresive effect by blocking the combination of IL 2 and IL 2R. In the liver transplanting model of rat, the injection of the peptide to the operated rat in five days would prolong the survival time of the rat.

Key words: Hexa peptide, Immunosuppressant, Phage peptide library, Liver transplant, Interleukin-2 receptor &alpha, chain

CLC Number:

O631

TrendMD:

SU Dan, SUN Zhao-Li, LI Yi, ZHOU Hui, LI Wei . The Biological Activities of the Small Peptide Immunosuppressant[J]. Chem. J. Chinese Universities, 2001, 22(10): 1744.

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The Biological Activities of the Small Peptide Immunosuppressant

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