Abstract:

Hybridization analysis is one of the most powerful methods of nucleic acid assays, including diagnostics of genetic disorders and infections of various etiologies. The biosensor detection allows an assay to be simplified and automatized^[1,2]. In this report a new electrochemical biosensing method based on coupling avidin-alkaline phosphatase(AV-AP) to the biotinylated oligonucleotide has been developed.