Studies on the Determination of Amino Acids by High Performance Liquid Chromatography with Laser Induced Fluorescence Intensified Charge Coupled Device Detector

Abstract

Abstract: A High performance liquid chromatography-laser induced fluorescence-intensified charge coupled device(HPLC-LIF-ICCD) system was used for the analysis of amino acid. Using 3-(4-carboxybenzoyl)-2-quinoline-carboxyaldehyde(CBQCA) as a derivatizing agent, several factors influencing the derivatization and separation of amino acid were investigated in detail. The mass detection limit of glycine was 5×10^-15 mol. The method was applied to the detection of amino acids in rat brain microdialysates.

Key words: High performance liquid chromatography, Laser induced fluorescence, Intensified charge coupled device, Separation, Microdialysis

CLC Number:

O657

TrendMD:

XIONG Shao-Xiang, HAN Hui-Wan, ZHAO Rui, WANG Guang-Hui, MA Hui-Min, CHEN Yi, LIU Guo-Quan . Studies on the Determination of Amino Acids by High Performance Liquid Chromatography with Laser Induced Fluorescence Intensified Charge Coupled Device Detector[J]. Chem. J. Chinese Universities, 2000, 21(8): 1191.

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Studies on the Determination of Amino Acids by High Performance Liquid Chromatography with Laser Induced Fluorescence Intensified Charge Coupled Device Detector

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