Studies on the Preparation and Characterization of Ethidium Bromide Labeled DNA Probe

Abstract

Abstract: The preparation and characterization of an ethidium bromide labeled DNAprobe is studied. Ethidium bromide was labeled onto a 21-bases 5'-oligonucleotides by EDC. The electrochemical characterization and fluorescence of the probe is discussed. The accumulation of sample DNAis also discussed. Besides it, the effect of base amounts on labeling and hybridization were discussed. We think the bases between 15—200 are better for electrochemical labeled DNAprobe. The EBlabeled DNAprobe was successfully used to detect sequence specific DNA. It shows a high sensitivity and selectivity. It is hoped for commodity use.

Key words: Ethidium bromide, Electrochemical label, DNA probe, Hybridization

CLC Number:

O657

TrendMD:

XU Chun, HE Pin-Gang, FANG Yu-Zhi . Studies on the Preparation and Characterization of Ethidium Bromide Labeled DNA Probe[J]. Chem. J. Chinese Universities, 2000, 21(8): 1187.

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Studies on the Preparation and Characterization of Ethidium Bromide Labeled DNA Probe

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