Identification of Major Metabolites of Etofesalamide in Rabbits by Liquid Chromatography/Ion Trap Mass Spectrometry

Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (5): 690.

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Identification of Major Metabolites of Etofesalamide in Rabbits by Liquid Chromatography/Ion Trap Mass Spectrometry

GU Jing-Kai¹, ZHONG Da-Fang², CHEN Xiao-Yan², ZHOU Hui¹, SHEN Jia-Cong¹, LIN Yang³

1. College of Life Science, Key Lab. for Supramolecular Structure and Spectroscopy, Jilin University, Changchun 130023, China;
2. Lab. of Drug Metabolism and Pharmacokinetics, Shengyang Pharmaceutical University, Shenyang 110015, China;
3. PLA Dalian Junior College of Medicine, Dalian 116017, China

Received:1999-08-30 Online:2000-05-24 Published:2000-05-24

Abstract

Abstract: The major metabolites of etofesalamide [N-(4-ethoxyphenyl)-2-hydroxyl-benzamide],a novel drug for acne therapy, in rabbits were described. Urine samples from 0 to 10 h were collected after single oral administration of 400 mg etofesalamide to four rabbits. The samples with or without hydrolysis by β-D-glucuronidase/sulphatase were purified by SPEcolumn (Sep-Pah C₁₈) and then were analyzed with LC/MSn for potential metabolites. Etofesalamide and its five metabolites were identified in rabbit urine based on the reference substances and mass spectra. These included oxaphenamide [N-(p-hydrox- yphenylsalicyl)] and the conjugates of glucuronide and sulfate for etofesalamide and oxaphenamide, respectively.

Key words: N-(4-Ethoxyphenyl)-2-hydroxyl-benzamide, Liquid chromatography/ion trap mass spectrometry, Metabolite

CLC Number:

O657.6

TrendMD:

GU Jing-Kai, ZHONG Da-Fang, CHEN Xiao-Yan, ZHOU Hui, SHEN Jia-Cong, LIN Yang. Identification of Major Metabolites of Etofesalamide in Rabbits by Liquid Chromatography/Ion Trap Mass Spectrometry[J]. Chem. J. Chinese Universities, 2000, 21(5): 690.

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Identification of Major Metabolites of Etofesalamide in Rabbits by Liquid Chromatography/Ion Trap Mass Spectrometry

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