Abstract: Amolecular templated synthetic polymer as the receptor highly selective for paracetamol was prepared by a molecular imprinting technique. The selective binding characteristics of the template polymer were evaluated by Scatchard analysis and Ultraviolet spectrometry. The results showed that the acrylamide as the functional monomer was more better than methacrylic acid for paracetamol selectivity, and a class of the same binding sites which could specially bind paracetamol by the interaction of hydrogen bonding were formed in the polymer and its dissociation constant was estimated to be 225×10^-3 mol/L. The selective binding experiment for substrates indicated that the polymer gave a higher affinity and selectivity for paracetamol. It is possible to be a good adsorption and binding material in the selective enrichment and determination of trace paracetamol in complex biosamples.

Key words: Molecular imprinting technique, Template polymer, Paracetamol, Substrates selectivity