Abstract: Amathematical model was deduced and described for the different mechanism of fluorescence quenching to analytes based on the essential quenching models including the dynamic quenching, the resonance energy transfer and the inner filter effects involving absorption of both excited light from the light source and emitted light from membrane. The model reported here indicated that the logarithmic functions of the intensity of fluorescence modulation in virtue of multiple quenching factors conform to the rule of addition. The response feature parameters of chemical sensor based on fluorescence quenching for analyses could be calculated by combining the multivariate linear model with a tri cup method. Aconception based on regarding the chemical sensor as the second source of light was advanced to get more useful information on analytes. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the chemical sensor to response a series of the therapeutic drugs. Atrain of thought for constructing the quantitative analysis model for the chemical membrane sensors based on the fluorescence quenching was afforded in the light of the experimental results.

Key words: Chemical sensors, Molecule probe, Fluorescence quenching, Multivariate linear model