Application of Resonance Raman Spectroscopy to Study the Effect of Rare-earth Ion Er3+on Myoglobin

Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (12): 1913.

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Application of Resonance Raman Spectroscopy to Study the Effect of Rare-earth Ion Er³⁺on Myoglobin

ZHAO Yu¹, XU Jin-Ze², ZHAO Da-Qing¹, XI Shi-Quan¹, ZHAO Bing², XU Wei-Qing²

1. State Key Laboratoryof Rare Earth Chemistryand Physics, Changchun Instituteof App lied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Key Laboratoryf or Supermolecular Structureand Spectroscopy, Jilin University, Changchun 130023, China

Received:2000-07-14 Online:2000-12-24 Published:2000-12-24

Abstract

Abstract: The effect of rare earth ion Er³⁺on myoglobin(Mb) was studied by using Resonance Raman spectroscopy.The results show that with the variation of Er³⁺concentrations, both the oxidation state and spin state of Mb are sensitive to the perturbation of Er³⁺. Er³⁺added to Mb affects the oxidation and spin state synchronously.The structure sensitive groups of Mb are more accessible to the Er³⁺than other groups.According to the fluorometry and CDspectra studied and our results as mentioned above, we considered that Er³⁺does not interact with heme directly, and Er³⁺probably leads to the conformational changes of Mb due to the change of oxidation and spin state of Heme

Key words: Rare-earth ion, Myoglobin, Oxidation state, Spin state, Resonance Raman spectroscopy

CLC Number:

O657.37

TrendMD:

ZHAO Yu, XU Jin-Ze, ZHAO Da-Qing, XI Shi-Quan, ZHAO Bing, XU Wei-Qing. Application of Resonance Raman Spectroscopy to Study the Effect of Rare-earth Ion Er³⁺on Myoglobin[J]. Chem. J. Chinese Universities, 2000, 21(12): 1913.

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Application of Resonance Raman Spectroscopy to Study the Effect of Rare-earth Ion Er³⁺on Myoglobin

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