Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (S1): 484.
• Biological Analysis • Previous Articles Next Articles
XIAO-Yao Xiao, DAVID McCalley
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Abstract:
A sensitive negative chemical ionization (NCI) gas-chromatography/mass spectrometry (GC/MS) method for the detection of estrogens is described. After hydrosis and clean-up by C18 ODS solid phase extraction (SPE) cartridge, the extracts obtained were derivatized with heptafluorobutyric anhydride and analyzed in the negative ion mode by GC/MS. Stability of derivatives was good. Selected ion monitoring (SIM) mode was applied to increase the sensitivity and, when possible, the higher m/z ions were selected to improve identification. The detection limit of the HFB esters in NCI using SIM was below 10 femtograms.
TrendMD:
XIAO-Yao Xiao, DAVID McCalley. High Sensitivity Negative Ion GC-MS Method for Detection of Estrogens in Urine[J]. Chem. J. Chinese Universities, 1999, 20(S1): 484.
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http://www.cjcu.jlu.edu.cn/EN/Y1999/V20/IS1/484
