Spectrophotometric Determination of Proteins by Using Nitrosulfophenol C

Abstract

Abstract: In this paper, it is found firstly that the nitrosulfophenol Ccan react with proteins to for MA pure blue complex in BrittonRobinson buffer solution at pH3.4 and room temperature, which gives maximuMAbsorption peak at 675 nm with 100 nm to red shift compared to that of nitrosulfophenol C. According to studies on optimum conditions, a new spectrophotometric method for determination of proteins was established. The standard working curves of eight kinds of proteins with larger linear ranges and higher sensitivity were determined. The new method was successfully applied to the determination of protein in human serum samples with satisfactory results in agreement with those of the classical coomassie brilliant blue G-250(CBB G-250) method.

Key words: Proteins, Nitrosulfophenol C, Spectrophotometry

CLC Number:

O657.32

TrendMD:

HU Qiu-Luan, LI Na, ZHAO Feng-Lin, LI Ke-An . Spectrophotometric Determination of Proteins by Using Nitrosulfophenol C[J]. Chem. J. Chinese Universities, 1999, 20(2): 221.

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Spectrophotometric Determination of Proteins by Using Nitrosulfophenol C

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