Studies on Kinetics of Reaction Between Tb 3+ and Human Oxyhemoglobin by Stopped-flow Fluorometry

Chem. J. Chinese Universities ›› 1998, Vol. 19 ›› Issue (6): 876.

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Studies on Kinetics of Reaction Between Tb ³⁺ and Human Oxyhemoglobin by Stopped-flow Fluorometry

CHENG Yi¹, LIN Hua-Kuan², WANG Kui³

1. Department of Chemistry, Nanjing University, Nanjing, 210093;
2. Department of Chemistry, Nankai University, Tianjin, 300071;
3. Department of Bioinorganic Chemistry, Beijing Medical University, Beijing, 100083

Received:1997-05-16 Online:1998-06-24 Published:1998-06-24

Abstract

Abstract: The kinetics of reaction between Tb ³⁺ and human oxyhemoglobin was investigated by means of stopped flow fluorometry. The Tb ³⁺ ions quenched the intrinsic fluorescence of oxyhemoglobin within the first 100 ms. The rate constant of the reaction at various temperature and activation parameters were obtained. The results indicate that Tb ³⁺ binding to oxyhemoglobin is attributed to the second order reaction. The mechanism of the reaction was explored.

Key words: Lanthanide, Oxyhemoglobin, Kinetics, Stopped-flow fluorometry

CLC Number:

O657.32

TrendMD:

CHENG Yi, LIN Hua-Kuan, WANG Kui. Studies on Kinetics of Reaction Between Tb ³⁺ and Human Oxyhemoglobin by Stopped-flow Fluorometry[J]. Chem. J. Chinese Universities, 1998, 19(6): 876.

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Studies on Kinetics of Reaction Between Tb ³⁺ and Human Oxyhemoglobin by Stopped-flow Fluorometry

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