Studies on the Reaction Mechanism and Properties of a New Peroxidase Fluorogenic Substrate Dihydro-2 (1H) -quinoxalone

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (7): 1035.

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Studies on the Reaction Mechanism and Properties of a New Peroxidase Fluorogenic Substrate Dihydro-2 (1H) -quinoxalone

GAO Jun, LI Yuan-Zong, HUANG Lan, WU Xin-Yuan, CI Yun-Xiang

Institute of Chemistry and Molecular Engineering, Peking University, Beijing 100871

Received:1997-03-04 Online:1997-07-24 Published:1997-07-24

Abstract

Abstract: Anew fluorogenic substrate, 3, 4-dihydro-2 (1H)-quinoxaloe (DHQXL), for per-oxidase was synthesized- It can be converted to strong fluorescent compound 2(1H)-quinox-alone(QXL) in the presence of horseradish peroxidase (HRP) or its mimetic enzymes heminand Mn-TPPS₄. Pure fluorescent product was isolated by HPLC.The structure of the prod-uct was determined using₁H NMRand MSspectroscopic techniques, which confirmed theproposed reaction mechanism for the enzyme catalyzed reaction.The property of the sub-strate was evaluated- The experimental results showed that it is a fluorogenic substrate forperoxidase that has a high potential in future application.

Key words: Fluorogenic substrate, 3,4-Dihydro-2 (1H)-quinoxalone, Horseradish peroxi-dase, Mimetic enzyme

CLC Number:

O621.1

TrendMD:

GAO Jun, LI Yuan-Zong, HUANG Lan, WU Xin-Yuan, CI Yun-Xiang. Studies on the Reaction Mechanism and Properties of a New Peroxidase Fluorogenic Substrate Dihydro-2 (1H) -quinoxalone[J]. Chem. J. Chinese Universities, 1997, 18(7): 1035.

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Studies on the Reaction Mechanism and Properties of a New Peroxidase Fluorogenic Substrate Dihydro-2 (1H) -quinoxalone

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