Non-enzymatic System for Catalytic Fluorometric Determination of Glucose Oxidase

Abstract

Abstract: Anovel non-enzymatic method for the determination of oxidases and their sub-strates was proposed.The principle was examined by fluorometric determination of glucose oxidase(GOD).Using EGTA (NH₄)₂Fe (SO₄), as modified Fenton reagent, H₂O₂, the prod-uct of GODcatalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GODwas thus measured according to the reaction rates.The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-

Key words: Glucose oxidase, Fluorometry determination, Hydroxyl radical

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LI Qing-Ge, XU Jin-Gou, HUANG Xian-Zhi, CHEN Guo-Zhen . Non-enzymatic System for Catalytic Fluorometric Determination of Glucose Oxidase[J]. Chem. J. Chinese Universities, 1997, 18(1): 52.

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Non-enzymatic System for Catalytic Fluorometric Determination of Glucose Oxidase

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