Abstract: Anew approach to resolve completely overlapping chromatographic peaks is developed in this paper.Taking the advantage of different disappearing rates of two components,the method can obtain the approximate spectrum of embedded component by first differentation of two-way data.Applying the method to resolving the two-way chromatograms with two overlapping peaks of ascorbic acid and nicotinic acid,the estimated spectra accord well with the pure ones.The result is satisfactory.

Key words: Chemometrics, Differentated two-way chromatography, Spectral and chromatographic resolution, Embedded peaks