Microscope Fluorescence-Hadamard Transform Three-Dimensional Image Analysis

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (6): 824.

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Microscope Fluorescence-Hadamard Transform Three-Dimensional Image Analysis

MEI Er-Wen, ZENG Xiao-Bin, CHEN Guan-Quan, ZENG Yun-E

Department of Chemistry, Wuhan University, Wuhan, 430072

Received:1993-09-07 Revised:1994-01-06 Online:1994-06-24 Published:1994-06-24

Abstract

Abstract: This research combines Hadamard transform multiplexed image technique with fluorescence microscopy.By this method, a Hadamard transform microscope cell fluorescence image was obtained with one detector(PMT)for the first time.The image shows a lot of useful information including the appearance ofcell, the content and the distribution of some species in a cell at same time.The result shows the possibility of using Hadamard multiplexed image technique to obtain a cell fluorescence image with one detector.

Key words: Hadamard transform, Fluorescence microscope, Image analysis

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MEI Er-Wen, ZENG Xiao-Bin, CHEN Guan-Quan, ZENG Yun-E. Microscope Fluorescence-Hadamard Transform Three-Dimensional Image Analysis[J]. Chem. J. Chinese Universities, 1994, 15(6): 824.

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Microscope Fluorescence-Hadamard Transform Three-Dimensional Image Analysis

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