Chemical Journal of Chinese Universities ›› 2020, Vol. 41 ›› Issue (6): 1127-1138.doi: 10.7503/cjcu20190663

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Single-molecule Imaging and Analysis of Signal Transduction Proteins on Cell Membranes

LIANG Yuxin,ZHAO Rong,LIANG Xinyue,FANG Xiaohong*()   

  1. Institute of Chemistry, Chinese Academy of Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, Beijing 100190, China
  • Received:2019-12-13 Online:2020-06-10 Published:2020-03-23
  • Contact: Xiaohong FANG
  • Supported by:
    † National Natural Science Foundation of China(21735006);National Natural Science Foundation of China(21890742);National Natural Science Foundation of China(21127901)


Membrane proteins play an important role in cell signal transduction. Structural and functional abnormalities of membrane proteins can cause a variety of diseases. The development of single-molecule fluorescence imaging technology has enabled researchers to study the location and dynamic behavior of single membrane proteins in living cell systems, and their interactions with other molecules. Single-molecule fluorescence imaging technology has been widely used to reveal the molecular mechanisms of signal transduction under physiological conditions in recent years. Based on the our research work, this paper introduces the imaging principle, fluorescent labeling method and data analysis method of single-molecule fluorescence imaging in the study of cell membrane proteins, and further reviews the research progress of single-molecule fluorescence imaging on mechanistic study of several important signal transduction membrane proteins and related drug development.

Key words: Single molecule fluorescence imaging, Cell membrane receptor, Total internal reflection fluorescence microscope, Super-resolution imaging, Single molecule analysis

CLC Number: