Studies on the Behavior of Urease Immobilized by Cross-Linked Polyvinyl Alcohol Gel

Chem. J. Chinese Universities ›› 1991, Vol. 12 ›› Issue (9): 1272.

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Studies on the Behavior of Urease Immobilized by Cross-Linked Polyvinyl Alcohol Gel

Pan Ji-lun, Chen Chang-zhi, Yu Yao-ting

Institute for Molecular Biology, Nankai Univeristy, Tianjin, 300071

Received:1990-06-22 Online:1991-09-24 Published:1991-09-24

Abstract

Abstract: The present paper reports the immobilization of urease on "cross-linked" PVAgel containing a high amount of water. Experimented results show that the immobilized urease on PVAgel has a high activity yield(79%). The immobilized urease activity is 63 mg/g (half hour at 37℃). The repeated determination with substrate showed no detectable loss in enzymatic activity. The determination of the activity within 26days at 37℃ showed a 26% loss of enzymatic activity.

Key words: PVA gel, Urease, Yield of enzymatic activity, Stability, Immobilization

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Pan Ji-lun, Chen Chang-zhi, Yu Yao-ting. Studies on the Behavior of Urease Immobilized by Cross-Linked Polyvinyl Alcohol Gel[J]. Chem. J. Chinese Universities, 1991, 12(9): 1272.

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Studies on the Behavior of Urease Immobilized by Cross-Linked Polyvinyl Alcohol Gel

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