Abstract: A new reversed-phase liquid chromatographic method with UV-detection for the analysis of amino acid-copper complex without any pretreatment or derivatizatiojn is studied. The mechanism of chromatographic separation and detection is described. The effect of factors such as the concentration of hexanesulfonate, copper ions and buffer as well as pHvalues is discussed. Guidelines are given for optimization of the separation. The analysis of amino acid samples are examined.

Key words: Complex of amino acid-copper, Reversed-phase liquid chromatography, UV-detection