Preparation of Enzyme Carriers with Regulated Solubility and Studies on Immobilized Enzyme

Abstract

Abstract: The methacrylic acid-acrylamide-maleic anhydride copolymers were prepared by radical precipitate copolymerization in dioxane. The copolymers exhibit the property of reversible precipitation-solubility changes due to their macromolecular complexes formed by hydrogen bonds when the pHvalues of the solution were changed. The immobilization of papain was carried out by using the copolymer as a carrier without other coupling agents. The immobilized papain shows the insoluble form below pH 3. 8 and soluble form above pH 3. 9. The immobilized papain with the advantages of soluble and in-soluble enzyme was obtained.

Key words: Macromolecular complex, Regulated solubility, Immobilized enzyme

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Li Zeng-ji, Shen Jia-cong, Ma Lin, Cheng Yu-hua. Preparation of Enzyme Carriers with Regulated Solubility and Studies on Immobilized Enzyme[J]. Chem. J. Chinese Universities, 1991, 12(5): 695.

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Preparation of Enzyme Carriers with Regulated Solubility and Studies on Immobilized Enzyme

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