Abstract: The microspheric phenol-formaldehyde copolymer, prepared by suspension polymerization from phenol and formaldehyde, was grafted by L-proline through 2-hydroxy propylene spacer to give a chiral chelating resin. Its copper(Ⅱ) chelate could be used as the stationary phase for the ligand-ex-change chromatography to resolve the racemates of proline, valine, isoleucine, methionine, histidine, asparagine,aspartic acid, and glutamic acid respectively.

Key words: Chiral chelating resin, Ligand-exchange chromatography, Resolution of DL-amino acids