Abstract: Anew method, Kalman filter fluoremetry, has been developed based on the characteristics and multicomponent Analysis model of conventional and synchronous fluorescence spectrometry. Several filtering procedures are examined and compared with each other. The tendency of state estimation, the reduction of error covariance and the irrelation of innovation sequence obtained from filtering process are investigated to be used as criteria to judge the assay reliability. The proposed method has been applied to determination of mixtures of the fluorescent dyes fluorescein, rhodamine Band rho-damine 6Gwith good results and with no separation.

Key words: Kalman filter fluoremetry, Synchronous and conventional fluorescence spectrometry, Fluorescent dyes, Multicomponent analysis, Chemometrics