Abstract: It was discovered that Cu(Ⅱ)-chloro complex ion appears accelera-tive effect to improve the chromatographic column effenciency of U(Ⅳ) species. For evaluating chromatographic characteristic was suggested a √lnC₀/C' ~t graphic method by which the accelerative effect of Cu(Ⅱ)-chloro ion under different conditions was discussed. Finally, the chromatographic separation of U(Ⅳ), Ti(Ⅳ) and Fe(Ⅲ) was realized by the help of the accelerative effect of Cu(Ⅱ)-chloro ion.