Abstract: Hydroxyapatite nanorods (nHAp) were synthesized using a solution precipitation method to mimic the inorganic composition of natural bone matrix, enhancing the osteoconductivity of the material. Concurrently, cobalt molybdenum phosphate microrods (CPMNr) were synthesized through a hydrothermal method. Their mediated Fenton-like reaction catalyzed the conversion of hydrogen peroxide into hydroxyl radicals, achieving effective tumor cell killing. A porous polyether ether ketone (PEEK) scaffold with continuous interconnected finger-like macropores and micropores was prepared using phase inversion to promote cell migration, nutrient exchange, and tissue ingrowth. CPMNr and nHAp were uniformly dispersed in a composite hydrogel precursor solution of methylacrylated oxidized hyaluronic acid (AHAMA) and polyethylene glycol methacrylate (PEGMA). These were grafted onto the porous PEEK scaffold surface through UV-initiated polymerization, endowing it with osteogenic activity and anti-tumor function. In vitro cell experiments demonstrated that the porous PEEK composite scaffold exhibits excellent biocompatibility with mouse embryonic osteoblast precursor cells (MC3T3-E1) and significantly enhances the expression levels of osteogenesis-related genes. Furthermore, the presence of CPMNr mediates a Fenton-like reaction within the tumor microenvironment, significantly elevating oxidative stress levels in human osteosarcoma cells (MG-63). This leads to reactive oxygen species accumulation and induces tumor cell apoptosis, demonstrating potent anti-tumor effect.

Key words: Hydroxyapatite；Cobalt molybdenum phosphate microrod；Porous poly ether ether ketone, Osteogenesis activity, Anti-tumor