Construction of GOx@Fe3O4-HNTs Microcapsule Reactor and Its Multi-enzyme Cascade Catalytic Performance

doi:10.7503/cjcu20230403

Abstract

Abstract:

The intracellular or intercellular microspace effectively enables various enzymatic reactions in organisms to proceed efficiently and orderly. Inspired from nature， this study combined the advantages of both natural enzymes and nanozymes to construct a microencapsulated reactor that simulates the enzymatic cascade reaction in vivo. First， the natural aluminosilicate mineral halloysite nanotubes（HNTs） were used as a carrier to in situ generate iron tetroxide（Fe₃O₄） with horseradish peroxidase-like activity on its surface. Then， it was used as a capsule wall material to encapsulate natural glucose oxidase（GOx）， forming the GOx@Fe₃O₄-HNTs microcapsule reactor. When glucose is added into the system， the GOx within microcapsule reactor will first convert the glucose into gluconic acid and hydrogen peroxide（H₂O₂）， and the H₂O₂ will continue to be catalyzed by Fe₃O₄ from the capsule wall and converted into hydroxyl radicals， triggering the color development of the substrate 3，3'，5，5'-tetramethylbenzidine（TMB）. Among them， Fe₃O₄-HNTs not only serves as the capsule wall material to protect GOx from environmental interference， but also can conduct a cascade catalytic reaction system with GOx. This kind of enzyme-nanozyme microencapsulation cascade system makes it have better catalytic performance and reaction stability than the natural enzyme systems. In addition， due to the participation of magnetic Fe₃O₄， the material is also recyclable and reusable. This enzyme-nanoenzyme composite microcapsule reactor provides a new method for simulating a multi-enzyme reaction system in the confined environment of organisms， and also lays a foundation for subsequent research in the fields of bioanalysis and biomimetic catalysis.

Key words: Nanoenzyme, Microcapsule reactor, Halloysite nanotube, Glucose oxidase, Cascade reaction

CLC Number:

O643.3

TrendMD:

HUANG Yuqing, LIU Yan, ZHANG Hongli, LIN Sen, SUN Shiyong, GOLUBEV Evgeny, LYU Rui, KOTOVA Olga, KOTOVA Elena. Construction of GOx@Fe₃O₄-HNTs Microcapsule Reactor and Its Multi-enzyme Cascade Catalytic Performance[J]. Chem. J. Chinese Universities, 2024, 45(1): 20230403.

Figures/Tables 12

References 28

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Catalyst	Substance	R²	K_m/（mmol∙L^-1）	v_max/（mmol∙mL^-1∙min^-1）
GOx	Glucose	0.993	831.6	1.5
Fe₃O₄	H₂O₂	0.996	159.4	0.3

Catalyst	Substance	R²	K_m/（mmol∙L^-1）	v_max/（mmol∙mL^-1∙min^-1）
GOx	Glucose	0.993	831.6	1.5
Fe₃O₄	H₂O₂	0.996	159.4	0.3

Catalyst	Substance	R²	K_m/（mmol∙L^-1）	v_max/（mmol∙mL^-1∙min^-1）
GOx@Fe₃O₄⁃HNTs	Glucose	0.979	99.94	0.38
GOx@Fe₃O₄⁃HNTs	TMB	0.993	1.95	0.35
GOx+HRP	Glucose	0.995	2.13	0.17
GOx+HRP	TMB	0.996	0.47	0.19

Catalyst	Substance	R²	K_m/（mmol∙L^-1）	v_max/（mmol∙mL^-1∙min^-1）
GOx@Fe₃O₄⁃HNTs	Glucose	0.979	99.94	0.38
GOx@Fe₃O₄⁃HNTs	TMB	0.993	1.95	0.35
GOx+HRP	Glucose	0.995	2.13	0.17
GOx+HRP	TMB	0.996	0.47	0.19

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Construction of GOx@Fe₃O₄-HNTs Microcapsule Reactor and Its Multi-enzyme Cascade Catalytic Performance

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