Fabrication and TXL Anti-cancer Drug Delivery of Magnetic Mesoporous Silica Nanoparticles†

doi:10.7503/cjcu20140122

Abstract

Abstract:

Magnetic Fe₃O₄@SiO₂ mesoporous silica nanoparticles(MSNs) were synthesized with Fe₃O₄ nanoparticles as crystal core, cetyltrimethylammium bromide(CTAB) as structural directional agent through the hydrolysis of tetraethoxysilane(TEOS) and self-assembly procedure under the basic conditions. The synthesized MSNs were characterized via the XRD, FTIR, TEM and N₂ adsorptiom-desorption. The results show that these MSNs have high sepcific surface area of 932 cm²/g, particles size of 150 nm and typical hexagonal packing mesoporous structure. Simultaneously, all Fe₃O₄ nanoparticles are perfectly embedded in silica bulk. As a TXL carrier, Fe₃O₄@SiO₂ exhibits good adsorption with the maximum adsorption capacity of 80 mg/g and TXL-Fe₃O₄@SiO₂ has a more than 120 h sustained release for TXL with accumulative quantity of 30 mg/g. While the release behaviour is controlled by various pH values. Higher the pH is, larger the accumulative release is. Experiments of cytotoxicity on HeLa cells indicate that the Fe₃O₄@SiO₂ particles exhibit excellent biocompatibility. TXL-Fe₃O₄@SiO₂ shows significant inhibition effect on HeLa cells than bare TXL under the same drug concentration.

Key words: Magnetic mesoporous nanoparticles, Paelitaxel(TXL), Anticancer drug delivery, HeLa cell, Fe3O4 nanoparticles

TrendMD:

ZHANG Zhuoqi, GENG Haoran, XUAN Ruifei, CHEN Minmin, CHEN Hui, LIU Aihui, CAO Xichuan. Fabrication and TXL Anti-cancer Drug Delivery of Magnetic Mesoporous Silica Nanoparticles^†[J]. Chem. J. Chinese Universities, 2014, 35(7): 1509.

Figures/Tables 8

Fig.1 TEM image of Fe3O4 nanoparticles

Fig.2 N2 adsorption-desorption isotherms and pore size istribution of Fe3O4@SiO2 nanoparticles

Fig.3 TEM image of Fe3O4@SiO2 nanoparticles

Fig.4 Small-angle XRD patterns of cal-Fe3O4@SiO2(a) nd pre-Fe3O4@SiO2 nanoparticles(b)

Fig.5 Wide-angle XRD patterns of Fe3O4@SiO2(a) nd Fe3O4 nanoparticles(b)

Fig.6 FTIR spectra of cal-Fe3O4@SiO2(a) nd pre-Fe3O4@SiO2(b)

Fig.7 Release of TXL from TXL-cal-Fe3O4@SiO2 various pH values in PBS at 37 ℃ ● pH=9.0; ▲ pH=7.3; ■ pH=5.5; ◆ pH=4.5. Samples were loaded at pH=7.3 with (80±5) mg/g loading capacity.

Fig.8 Effect of cal-Fe3O4@SiO2, TXL-Fe3O4@SiO2 and TXL with various amounts on the relative HeLa cells viability

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