Synthesis and Proporties of Temperature and pH-sensitive and Gadolinium Contained Polymeric Magnetic Resonance Imaging Contrast Agent†

doi:10.7503/cjcu20150663

Chem. J. Chinese Universities ›› 2016, Vol. 37 ›› Issue (1): 155.doi: 10.7503/cjcu20150663

• Polymer Chemistry • Previous Articles Next Articles

Synthesis and Proporties of Temperature and pH-sensitive and Gadolinium Contained Polymeric Magnetic Resonance Imaging Contrast Agent^†

LIU Ruiqing¹, LIANG Shuang², JIANG Cun¹, XU Zushun^1,^*(), XU Haibo^2,^*

1. Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials; Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, China
2. Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Received:2015-08-20 Online:2016-01-10 Published:2015-12-20
Contact: XU Zushun,XU Haibo E-mail:xuhaibo1120@hotmail.com
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.51273058, 81171386, 81372369)

Abstract

Abstract:

Paramagnetic, temperature and pH-sensitive polymeric particles were prepared with N-isopropylacrylamide, methacrylic acid, styrene, and polymerizable Gd(AA)₃phen·H₂O using emulsifier-free emulsion polymerization technique. Dynamic light scattering result shows that the diameter of the polymeric particles changes by about 60 nm as a result of variations in either the temperature or pH, indicating that the particles are pH and temperature-sensitive. The T₁-weighted relaxivity values at 3 T are about 11.3 L/(mmol·s) which is 2.6 times higher than clinical Magnevist^?. In vivo MR imaging reveals that the TPRPPs can be effectively imaged in the liver and spleen, suggesting that they are excellent magnetic resonance imaging agents.

Key words: Temperature and pH-sensitive polymer, Magnetic resonance imaging(MRI), Emulsifier-free emulsion polymerization, Gadolinium contrast agent

CLC Number:

O633

TrendMD:

LIU Ruiqing, LIANG Shuang, JIANG Cun, XU Zushun, XU Haibo. Synthesis and Proporties of Temperature and pH-sensitive and Gadolinium Contained Polymeric Magnetic Resonance Imaging Contrast Agent^†[J]. Chem. J. Chinese Universities, 2016, 37(1): 155.

Figures/Tables 7

Scheme 1 Synthetic routes of TPRPP

Fig.1 FTIR spectra of Gd(AA)3phen·H2O(a), TPRPP1(b) and TPRPP(c)

Fig.2 TEM images of TPRPP1(A) and TPRPP(B)

Fig.3 Dh of TPRPP as function of temperature(A), pH(B), variation in Dh of TPRPP as alternate temperature of 25 and 37 ℃(C) and alternate pH value of 2.0 and 7.4(D)^ The pH of (A) and (C) is 6.0; the temperature of (B) and (D) is 25 ℃.

Fig.4 Cell viability against Hela cells assessed by standard MTT assay

Fig.5 T1-weighted relaxivity of TPRPP^The longitudinal relaxation time(T1) is measured on a clinical 3.0 T MR scanner at various Gd(Ⅲ) concentrations.

Fig.6 T1-weighted MR images taken at different time points^T1/min: (A) 0; (B) 15; (C) 30; (D) 60; (E) 120; (F) 360.

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Synthesis and Proporties of Temperature and pH-sensitive and Gadolinium Contained Polymeric Magnetic Resonance Imaging Contrast Agent^†

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