Rapid Measurement of Synthetic Pigments by Thermal Desorption-High Field Asymmetric Waveform Ion Mobility Spectrometry(FAIMS) Technology†

doi:10.7503/cjcu20140139

Abstract

Abstract:

Field asymmetric ion mobility spectrometry(FAIMS) technique was introduced into the sensitive and rapid analysis of four synthetic pigments for the first time. The chrysoidin, malachite green, rhodamine B and methyl red were detected by a co-developed thermal desorption-FAIMS(TD-FAIMS) system. The ion characteristic signals of synthetic pigments were acquired under the optimized conditions with the injection temperature of 160 ℃ and the carrier gas flow rate of 1.6 L/min. Compensation voltage(CV) values with different modes were adopted for qualitative measurement. FAIMS detection signals in positive mode appeared at CV=0.41 and 0.89 V for chrysoidine and rhodamine B, respectively, while negative mode detection signals of methyl red and malachite green appeared at CV=0.67 and 0.02 V, respectively. Using external stan-dard method for quantitative measurement, the linear relationship(R²) was above 0.9967. The limits of detection(LOD) were 2.5—10 μg/L, and limits of quantification(LOQ) were 5—20 μg/L, respectively. The average recoveries were higher than 78.2% and the relative standard deviation(RSD) was less than 8.0%. This study laid a foundation for the application of FAIMS technique to rapid detection of synthetic pigments. The FAIMS instrument, which are highly sensitive, simple and fast responsive, demonstrated huge potential in the rapid analysis of synthetic pigments.

Key words: High-field asymmetric waveform ion mobility spectrometry(FAIMS), Synthetic pigment, Chrysoidin, Malachite green, Rhodamine B, Methyl red

CLC Number:

O656.31

TrendMD:

LI Juan, ZHAO Weijun, LI Lingfeng, LI Xin, LI Peng, WANG Xiaozhi. Rapid Measurement of Synthetic Pigments by Thermal Desorption-High Field Asymmetric Waveform Ion Mobility Spectrometry(FAIMS) Technology^†[J]. Chem. J. Chinese Universities, 2014, 35(7): 1403.

Figures/Tables 8

Fig.1 Principle of high-field asymmetric waveform ion mobility spectrometer

Fig.2 Photo(A) and schematic diagram(B) of FAIMS chip (B) L represents the separation channel length(300 μm) and g is the separation channel gap(35 μm).

Fig.3 Chemical structures of four pigments

Fig.4 Effects of the headspace sample temperature(A) and carrier gas flow rate(B)

Fig.5 FAIMS spectra of chrysoidin(A) and rhodamine B(B) and their positive mode comparison diagram(C), FAIMS spectra of malachite green(D) and methyl red(E) and their negative mode comparison diagram(F)

Table 1 Linear range, correlation coefficient, regressin equation, LOD and LOQ of four synthetic pigments

Synthetic pigment	Linear range/ (μg·L^-1)	Correlation coefficient, R²	Regression equation	LOD^a/(μg·L^-1)	LOQ^b/(μg·L^-1)
Malachite green	5—100	0.9967	y=0.0075x+0.0061	2.5	5
Rhodamine B	10—200	0.9996	y=0.0049x+0.0029	5	10
Methyl red	20—400	0.9989	y=0.0051x-0.0171	10	20
Chrysoidin	5—100	0.9993	y=0.0015x+0.0469	2.5	5

Table 2 Spike recoveries and RSDs(n=6) of this method

Synthetic pigment	Added/(μg·kg^-1)	Measured/(μg·kg^-1)	Recovery(%)	RSD(%, n=6)
	50	39.1	78.2	4.78
Malachite green	250	201.3	80.5	3.33
	500	396.0	79.2	3.02
	50	37.7	75.4	4.57
Rhodamine B	500	395.0	79.0	4.36
	1000	820.0	82.0	2.75
	100	80.8	80.8	7.68
Methyl red	500	402.5	80.5	4.32
	1000	798.0	79.8	1.61
	50	41.4	82.7	5.87
Chrysoidin	250	204.8	81.9	5.03
	500	399.5	79.9	2.73

Fig.6 FAIMS spectra of red tea sample(A) and added chrysoidin(B)and their comparison diagram(C)

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