Thermodynamic Studies on Catalytic Reaction Between Bromelain and Soybean Protein

doi:10.7503/cjcu20130292

Abstract

Abstract:

The power-time curves of reaction between bromelain and soybean protein were determined using microcalorimeter. Using the thermodynamic theory and reduced extent method, the Michaelis constant(K_m) and the maximum velocity(V_max) of catalytic reaction were obtained at different temperatures and pH values, and the equations from the relation between K_m and temperatures(K_m-T), and pH values(K_m-pH) were established. Based on the established equations, the optimum temperature(314.63 K) and the optimum pH(6.99) were gained. Under the optimum temperature and pH, the power-time curves of catalytic reaction with metal ions were obtained, and the apparent Michaelis constant(K_m') and the maximum reaction velocity(V_max') were gained. The relations between the K_m' and the contents of metal ions were established. Compared with the K_m and V_max, the activation or inhibition effect of metal ions on enzymatic reaction could be known.

Key words: Microcalorimetric method, Thermodynamic, Bromelain, Catalytic reaction, Soybean protein

CLC Number:

O642.3

TrendMD:

LI Xiang-Yang, DONG Hai-Zhou, ZHANG Hong-Lin. Thermodynamic Studies on Catalytic Reaction Between Bromelain and Soybean Protein[J]. Chem. J. Chinese Universities, 2013, 34(12): 2861.

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