Correlation of Enolase and the Maturation of SpeB in Streptococcus pyogenes

doi:10.7503/cjcu20120794

Abstract

Abstract:

Group A Streptococci(GAS) is an important Gram-positive human pathogen, which causes mild to severe diseases. The ability of GAS to cause infection is associated with the production of an array of secreted and cell-wall associated virulence factors. One key secreted virulence factor is a cysteine protease called streptococcal pyrogenic exotoxin B(SpeB). This protein is initially secreted as a 40000 precursor zymogen, and is subsequently cleaved to a 28000 mature form. The conversion of the zymogen form of SpeB is extremely complex and unclear. In this paper we reported a protein involved in the maturation of SpeB in the supernatant of Group A Streptococci. This protein was the streptococcus host plasminogen receptor, enolase(Eno) identified by MS/MS analysis. We constructed the eno in-deletion mutants by the method of gene knock out and investigated the correlation of enolase and maturation of SpeB in GAS. Western blot analysis and protease assays revealed a delay in the maturation of SpeB in supernatant of the eno in-deletion mutants comparing with the wild type strains. Far-Western blot analysis showed that SpeB can bind to Eno, which interact with each other. These data imply that the function of Eno, as like HtrA and RopA, is a chaperone, the regulation of protease activity and the mechanism of secretion in GAS.

Key words: Enolase, Streptococcal pyogenic exotoxin B, Eno in-deletion mutant, Streptococcus pyogenes

CLC Number:

TrendMD:

HU Shan, MA Chao, LI Xue-Ru, LI Ming, LIU Yan-Hong, JIANG Nan-Ping, GUO Tai-Lin, YAO Ning. Correlation of Enolase and the Maturation of SpeB in Streptococcus pyogenes[J]. Chem. J. Chinese Universities, 2013, 34(4): 886.

References

[1] Debra E. B., Infection, Genetics and Evolution, 2009, 9(4), 581—593

[2] Carroll R. K., Musser J. M., Mol. Microbiol., 2011, 81(3), 588—601

[3] Zhang B., Li X. R., Jiang N. P., Ren Y. Y., Chinese J. Infect. Control, 2012, 11(4), 316—318(张波, 李学如, 江南屏, 任瑶瑶. 中国感染控制杂志, 2012, 11(4), 316—318)

[4] Lyon W. R., Caparon M. G., J. Bacteriol., 2003, 185(12), 3661— 3667

[5] Lyon W. R., Caparon M. G., Infect. Immun., 2004, 72(3), 1618—1625

[6] Li X. R., Li Y., Wang Y., Yao N., Guo T. L., Meng T., Chinese Journal of Laboratory Medicine, 2009, 32(6), 699—700(李学如, 李尧, 王艳, 姚宁, 郭泰林, 孟涛. 中华医学检验杂志, 2009, 32(6), 699—700)

[7] Li Y., Lan X. L., Li X. R., Guo T. L., Yao N., Acta Microbiological Sinica, 2010, 50(6), 822—827(李尧, 蓝小玲, 李学如, 郭泰林, 姚宁. 微生物学报, 2010, 50(6), 822—827)

[8] Scot R. W., Rosa I. V., Patrick H., Electrophoresis, 2002, 23(18), 3182—3192

[9] Ma M. X., Ma C., Cheng J., Xie Z. S., Li M., Fei D. L., Chem. J. Chinese Universities, 2011, 32(8), 1768—1773(马鸣潇, 马臣, 程健, 谢振声, 李明, 费东亮. 高等学校化学学报, 2011, 32(8), 1768—1773)

[10] Fisher M., Huang Y. S., Li X. R., McIver K. S., Toukoki C., Eichenbaum Z., Infect. Immun., 2008, 76(11), 5006—5015

[11] Cole J. N., Aquilina J. A., Hains P. G., Henningham A., Sriprakash K. S., Caparon M. G., Nizet V., Kotb M., Cordwell S. J., Djordjevic S. P., Proteomics, 2007, 7(24), 4488—4498

[12] Hytonen J., Haataja S., Gerlach D., Podbielski A., Finne J., Mol. Microbiol., 2001, 39(2), 512— 519

[13] Hall R. A., Methods Mol. Biol., 2004, 261, 167—174

[14] Yuya S., Masafumi K., Hiroyuki A., Masaharu I., Yasuo I., J. Biosci. Bioen., 2011, 112(3), 304—307

[15] Cork A. J., Jergic S., Hammerschmidt S., Kobe B., Pancholi V., Benesch J. L., Robinson C. V., Dixon N. E., Aquilina J. A., J. Biol. Chem., 2009, 284(25), 17129—17137

[16] Kang S. O.,Caparon M. G., Cho K. H., Infect. Immun., 2010, 78(6), 2754—2767

[17] Chuan C. N., Wu J. J., J. Formos Med. Assoc., 2008, 107(9), 677—685

[18] Nyfeler B., Michnick S. W., Hauri H. P., Proc. Natl. Acad. Sci., 2005, 102(18), 6350—6355

[19] Rosch J., Caparon M., Science, 2004, 304(5676), 1513—1515

[20] Hauser A. R., Schlievert P. M., J. Bacteriol., 1990, 172(8), 4536—4542

[21] Wang X. D., Li Z. L., Acta Biophysica Sinica, 2009, 25(3), 157—167(王晓东, 李智立. 生物物理学报, 2009, 25(3), 157—167)