Abstract: Ordered mesoporous SBA-15 silica spheres were synthesized using the triblock copolymer, Pluronic P123, as the template with the assistance of KCl and 1,3,5-trimethylbenzene in the acidic condition. The obtained SBA-15 silica spheres with the particle size of 4.5 μm, pore size of 22.8 nm, surface area of 546.99 m²/g, and pore volume of 1.747 m³/g were used as the support in high-performance liquid chromatography(HPLC). The 3,5-dimethylphenylcarbamates of cellulose bearing about 3% of 3-(triethoxysilyl)propyl residues was immobilized onto SBA-15 particles by intermolecular polycondensation of the triethoxysilyl groups as the chiral stationary phase(CSP). The CSP was evaluated by HPLC for ten chiral alcohols with the eluents containing chloroform and tetrahydrofuran, which cannot be used with the conventional coated-type CSPs. Using the eluents, this CSP showed higher chiral recognition abilities to compare with that CSP based the same cellulose derivative bonded onto silica gel. The results indicated that the mesoporous SBA-15 spheres could be used as a good candidate for HPLC packings.

Key words: Mesoporous SBA-15 silica spheres, Cellulose-tirs(3,5-dimethylphenylcarbamate), Bonded chiral stationary phase, Enantioseparation