高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

介孔材料的修饰及固定青霉素酰化酶的稳定性研究

高波1,2, 朱广山1, 付学奇2, 滕利荣2, 陈静1, 王春艳1, 裘式纶1   

    1. 吉林大学无机合成与制备化学国家重点实验室,
    2. 生命科学学院, 长春 130012
  • 收稿日期:2005-07-06 修回日期:1900-01-01 出版日期:2006-10-10 发布日期:2006-10-10
  • 通讯作者: 裘式纶

Modification of Mesoporous Material and Stability of Immobilization of Penicillin G Acylase

GAO Bo1,2, ZHU Guang-Shan1, FU Xue-Qi2, TENG Li-Rong2, CHEN Jing1, WANG Chun-Yan1, QIU Shi-Lun1   

    1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,
    2. College of Life Science, Jilin University, Changchun 130012, China
  • Received:2005-07-06 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
  • Contact: QIU Shi-Lun

PDF (PC)

被引次数

23

摘要： 利用扩孔剂的作用合成出较大孔径(12 nm)的介孔材料SBA-15, 并进行表面氨基修饰, 以此为载体, 以戊二醛为交联剂, 对青霉素酰化酶进行组装固定, 并对固定化青霉素酰化酶(PGA)的稳定性进行了深入的研究. 实验结果表明, PGA与载体交联后仍保持活性. 热稳定性研究结果表明, 制备的固定化青霉素酰化酶在低于60 ℃时保持稳定; pH在6~11范围内保持稳定; 固定化酶重复使用10次之后, 仍具有高达90%的残留活力.

关键词: 介孔材料, 青霉素酰化酶, 稳定性

Abstract: Mesoporous materials of large pore size(12 nm)were synthesized by the usage of swelling agent. After modification, the mesoporous materials were used as the carriers in the immobilization of penicillin G acylase. Specifically, a study was carried out on the stability of the immobilized enzyme. Penicillin G acylase can catalyze the cleavage of the amide bond in the benzyl penicillin(penicillin G) side-chain to yield phenylacetic acid and 6-aminopenicillanic acid(6-APA). The enzyme is of great pharmaceutical importance, as the product 6-APA is the starting point for the synthesis of many semi-synthetic penicillin antibiotics. The activity of nature PGA is low, which limits the application of PGA. By immobilizing PGA on a solid support, it can be reused and its useful lifetime can be extended because the immobilized PGA is less susceptible to degradation, aggregation, or denaturation.The research results indicate that the functionalization of the internal surfaces of mesoporous solids permitted the enzyme immobilization. The reuse of the catalysts is also possible. The retention activity up to 90% of the PGA is observed in the most favorable case after using for 10 times.

Key words: Mesoporous materials, Penicillin G acylase, Stability

中图分类号: 

TrendMD: