高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

胃蛋白酶对CdTe纳米粒子的表面修饰及分析应用

陈莉华1,2, 覃事栋1, 李朝阳3   

    1. 吉首大学化学化工学院, 吉首 416000;
    2. 湖南大学化学生物传感与计量学国家重点实验室, 长沙 410082;
    3. 吉首大学生物资源与环境科学学院, 吉首 416000
  • 收稿日期:2007-06-11 修回日期:1900-01-01 出版日期:2008-02-10 发布日期:2008-02-10
  • 通讯作者: 陈莉华

Surface-modification of CdTe Nano-particles by Pepsin and Its Analytical Application

CHEN Li-Hua1,2*, QIN Shi-Dong1, LI Zhao-Yang3   

    1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China;
    2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China;
    3. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China
  • Received:2007-06-11 Revised:1900-01-01 Online:2008-02-10 Published:2008-02-10
  • Contact: CHEN Li-Hua

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被引次数

18

摘要： 以巯基乙酸为稳定剂和表面修饰剂, 在有机相中合成了平均粒径为3 nm左右的CdTe纳米粒子, 用胃蛋白酶改变纳米粒子的表面修饰状态并研究其系列特性. CdTe纳米粒子在320 nm处有强的紫外吸收, 在524.8 nm处有荧光发射. 经胃蛋白酶对其表面修饰后, 紫外吸收峰位不变, 但吸光强度升高, 荧光峰位蓝移至467.2 nm, 荧光强度降低. 温度、pH值及离子强度均对表面修饰产生影响. 在最佳实验条件下, 胃蛋白酶质量浓度在4—40 mg/L范围内与荧光降低值之间呈线性关系, 检测限(3σ)为0.28 mg/L(n=10), 该方法已被用于人体胃液胃蛋白酶的测定.

关键词: CdTe纳米粒子, 胃蛋白酶, 表面修饰, 荧光测定

Abstract: CdTe semiconductor nano-particles were synthesized with mercaptoethylic acid as the stabilizer as well as surface-modification reagent. The average size of CdTe nano-particles estimated from TEM graph was ca. 3 nm. The CdTe nano-particles exhibited a strong ultraviolet absorption at 320 nm and fluorescence emission at 524.8 nm, respectively. When surface-modification of CdTe nano-particles were replaced with pepsin, ultraviolet absorption peak kept the same, absorption intensity at 320 nm increased. It can be observed that the fluorescence emission intensity decreased as well as the fluorescence peak shifted to 467.2 nm. Temperature, ion strength and pH value influenced the surface-modification of CdTe nano-particles. Under the optimal conditions, a mass concentration of pepsin in the range of 4—40 mg/L shows a linear relationship with the fluorescence intensity decreasing value of CdTe nano-particles, with a detection limit 3σ of 0.28 mg/L(n=10). The proposed method was applied to detection of the concentration of pepsin in human gastric juice.

Key words: CdTe nano-particles, Pepsin, Surface-modification, Fluorescence determination

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